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العنوان
Micropropagation of some Pot Plants /
المؤلف
Salem, Mohamed Mohsen Mohamed Abass.
هيئة الاعداد
باحث / محمد محسن محمد عباس سالم
مشرف / عبـد العزيز كامل ضـوه
مشرف / هشام عبد العال عبد المنعم الشامي
مشرف / سوسن سامي سيد عبد الرحمـن
الموضوع
Plant micropropagation.
تاريخ النشر
2016.
عدد الصفحات
161 p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البساتين
تاريخ الإجازة
1/1/2016
مكان الإجازة
جامعة الزقازيق - كـليـــة الزراعـــة - البساتين
الفهرس
Only 14 pages are availabe for public view

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Abstract

In order to establish in vitro micropropagation protocol for Aglaonema commutatum and Alocasia cucullata, different concentrations (0.0, 1.0, 2.0, 4.0, 8.0 or 16.0 mg/l) of cytokinins (benzyladenine, kinetin or isopentenyladenine) and the combinations between the best cytokinin treatment and different concentrations (0.0, 0.5, 1.0 or 2.0 mg/l) of auxins (indoleacetic acid or naphthaleneacetic acid) were investigated. Also, paclobutrazol (0.0, 0.5, 1.0, 2.5 or 5.0 mg/l), casein hydrolysate (0.0, 0.5, 1.0, 2.0 or 4.0 g/l), sucrose (20, 30, 40 or 50 g/l), progesterone (0.0, 1.0, 2.0, 4.0, 8.0 or 16.0 mg/l), malt extract (0.0, 1.0, 2.0, 4.0 or 8.0 g/l) and diphenylurea (0.0, 0.25, 0.5, 1.0 or 2.0 mg/l) were tested in combinations with the best cytokinin/auxin treatment during multiplication stage for both experimental plants. It was found that the best multiplication rates (6.70 and 19.10 shoot/explant) for Aglaonema commutatum and Alocasia cucullata were obtained with 8.0 mg/l BA + 0.5 mg/l NAA and 8.0 mg/l BA + 1.0 mg/l IAA + 4.0 mg/l progesterone, respectively. During rooting stage different concentrations (0.0, 0.25, 0.5, 1.0 or 2.0 mg/l) of indole-3-acetic acid (IAA), naphthaleneacetic acid (NAA) or indole-3-butyric acid (IBA) were investigated. Recorded data indicated that 1.0 mg/l NAA was the most suitable treatment for rooting stage for both species. Rooted shoots obtained from rooting stage were acclimatized by transferring them to plastic pots containing peat moss, vermiculite, perlite or sand each alone or in different combinations between them at equal volume proportions. Results referred that perlite and peat moss at equal volume proportions seems to be the best medium for Aglaonema commutatum plantlets acclimatization, while perlite: peat: vermiculite, peat: vermiculite or peat: sand at equal volume proportions were the most suitable media for Alocasia cucullata plantlets acclimatization.