الفهرس 
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Abstract
Paraoxonase enzyme-1 is an estrase enzyme leading to reduction in oxidative stress in serum lipoprotiens. PON-1 (E.C.3.8.1) synthesis in the liver and secreted in the blood where associated with HDL-c. PON-1 protect both HDL-c and LDL-c against lipid perioxidationand prevention of atherosclerosis. In human, PON-1 have tow coding region polymorphism, Q192R has been widely investigated as amino acid R192 is an important active site residue.
The aim of this study was to investigate paraoxonase, arylestrase and glutathione perioxidase activiteis and malondialdehide level. Also determination of serum lipid profile and serum protein level and activiteis of AST, ALT and investigate of PON-1 R192 gene in cattle and investigate the serum PON-1 activity in normal and oxidative stress cows and comparison between the two groups.
This study was carried on a total number of 28 Friesian Holstein cows as follows:
● group one: consist of 14 normal cows (control cows).
● group two: consist of 14 oxidative stress cows (parasitic and mastitic cows).
These animals were under same condition in feed and environmental condition and lactating age.
Two blood samples were collected. One for serum separated for biochemical determination, second for Buffy coat separated for DNA amplification.
Separated serum was subjected to biochemical analysis.
Our study revealed the following results:
1. Bovine paraoxonase genedoes not code region inR-192 genotype such as in human PON gene.
2. The protein sequence similarity between two species gene showed that, the cattle PON1 protein covered 82% of human protein.
3. The DNA amplification of PON1 in infected animal was significantly higher than that of healthy animal.
4. Serum PON1 activity was significantly decrease in oxidative stress cows.
5. Serum PON-1activity had a positive correlation withArylestrase activity in oxidative stress cows. Also, PON-1 had a positive correlation with GPX activity in oxidative stress cows.
6. PON-1 activity had a negative correlation with MDA level in oxidative stress cows.
7. GPX activity had a negative correlation with MDA production in oxidative stress.
8. Lipid profile were similar in oxidative stress cows and normal one.
9. Albumin level was decreased in oxidative stress cows, while total serum protein and Globulin level was unchanged in both groups. A∕ G was increased in oxidative stress cows.
10. AST was increased in oxidative stress cows while ALT was decreased when compared to normal ones.