الفهرس 
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Abstract
• Monomicrobial non neutrocytic bacterascites (MNB) was the most prevalent variant of spontaneous bacterial peritonitis.
• Inoculation of ascitic fluid into blood culture bottles significantly improves culture positivity rate (20.7%) for diagnosis of SBP compared to conventional culture technique (12.1%). While the positive diagnostic yield was 9.3% and 7.9% when culturing on blood agar supplemented with Tween 80 and culture of samples treated with Triton X-100 on blood agar, respectively.
• There was a good agreement between conventional culture results and either culture on blood culture bottles, culture on blood agar supplemented with Tween 80 or culture of samples treated with Triton X-100 on blood agar.
• By conventional culture, culture on blood culture bottles and culture on blood agar supplemented with Tween 80, the most common isolated organism was Staphylococcus coagulase negative, whereas Staph. aureus and Enterococci were the most commonly isolated organisms by culture of samples treated with Triton X- 100 on blood agar.
from this study, the following is recommended:
• Diagnostic abdominal paracentesis for cell count and culture should be performed for any patient with ascites to detect spontaneous bacterial peritonitis or one of its variants in view of the high prevalence of this complication in such patients, especially those with more advanced liver disease.
• The ascitic fluid should be referred to the laboratory in a sterile tube containing an anticoagulant (usually EDTA) to avoid clotting and allow for cell count. In hemorrhagic ascites, peripheral blood tests should be performed over processing of ascitic fluid cell count for correction of white blood cell and neutrophil counts.
• Conventional culture and blood culture bottles remain the best methods for diagnosing spontaneous bacterial peritonitis.
• Further studies should be done with larger number of samples for evaluation of the role of Tween 80 and Triton X-100 in diagnosis of ascitic fluid infection.