الفهرس | Only 14 pages are availabe for public view |
Abstract The current study was aimed to investigate the possible use of clover (Trifolium alexandrinum) plant (flowers, leaves, stems, roots and seeds) on CCl4 - intoxicated rats. For this purpose, the study included (35) rats (150±10 g) weight. Rats were fed on normal (basal) diet. The animals were divided to 7 equal group, one was kept as control negative (-ve) group, while the other 6 groups were injected by carbon tetrachloride in olive oil 50% v/v (3 ml/kg .B.wt) twice a week for 14 days to induce hepatotoxicity . group (2) : Control positive (+ve), in which hepatotoxic rats Were injected by(CCl4) and fed on basal diet for 28 days. group (3): CCl4 hepatotoxicity rats were fed on basal diet containing 5% clover flowers for 28 days. group (4):CCl4 hepatotoxicity rats were fed on basal diet containing 5% clover leaves for 28 days. group (5): CCl4 hepatotoxicity rats were fed on basal diet containing 5% clover stems for 28 days. group (6): CCl4 hepatotoxicity rats were fed on basal diet containing 5% clover roots for 28 days. group (7): CCl4 hepatotoxicity rats were fed on basal diet containing 5% clover seeds for 28 days. At the end of the experimental (28 days), animal were scarified. Blood samples were collected to determine the fullowing parameters : Blood glucose, lipids fraction of serum (TC,TG, HDL-c, LDL-c, VLDL-c, AI), serum liver enzymes (AST, ALT, ALp, AST/ALT ratio), serum protein fractions (total protein, albumin, globulin, albumin/globulin ratio), serum bilirubin fractions (total bilirubin, direct bilirubin, indirect bilirubin) , antioxidant enzymes (GPX , SOD, CAT) & kidney function (creatinine, urea, uric acid). Liver, kidney, lungs, heart and spleen were kept on (10%) formalin solution for histopathological examination. |