الفهرس 
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Abstract
Banana (Musa spp. family Musaceae) is a fourth most important fruit crop in the world. The present study was carried out in the plant tissue culture laboratory and greenhouse in the Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia during the period 2013 – 2015. The explants were collected from suckers grown around banana fruiting mother plants, cultivar Grande Naine. The present investigation aimed to identify the effect of media type (solid or liquid) supplemented with cytokinin or NAA with or without AC and light intensity, on the different stages of banana micropropagation. The present study revealed that medium supplemented with 3 mg/l BAP in liquid media produced greater number of shoots (7.20) and shoot length (7.40 cm) on solid MS medium suplemented 0.0 mg/l BAP with 5 mg/l Kin compared with other treatments. The highest number of roots (14.40) was observed in medium supplemented with 3 mg/l NAA, AC under 3000 Lux light intensity. Survival rate of the plantlets were found to be 100% during first acclimatization and 60 – 100% at the second acclimatization in the greenhouse.
Key words: Banana, Musa spp., Tissue culture, micropropagation, cytokinin, media types, shoots, roots, plantlets, acclimatization.