الفهرس 
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Abstract
The present study was conducted at the tissue culture lab. Center of Genetic Engineering and Biotechnology, Fac. Agric., Ain Shams University, Egypt during the period from 2013 to 2015 to propagate strawberry (Fragaria x ananassa Duch) cultuvars,i.e. Festival and Marquez through tissue culture technique. Sterilized runner tips were cultured on Murashige and Skoog (MS) medium. This work included four experiments and the results showed the following: First experiment: Investigated the micropropagation of Festival and Marquez strawberry cultivars by using MS medium supplemented with different concentration of gibberellic acid (GA3), i.e.(0.1, 0.2, 0.3, 0.4 and 0.5 mgl-1) in multiplication stage. Data cleared that Festival cv. amended with 0.4 mgl-1 was the best for giving the highest number of shoots /explant and number of leaves/shoot during multiplication stage. Second experiment: Aimed to the effect of sucrose concentrations (15, 20, 25, 30 and 35 gl-1 ) applied to MS medium during multiplication phase through micropropagate the strawberry Festival and Marquez cvs. Data show that , both the two used strawberry cultivars produced the maximum values of number of shoots/ explant, number of leaves/ shoot and shoot length at 35 gl-1. Third experiment: Show the effect of two IBA concentration (0.1 and 0.5 mgl-1) applied to MS medium. In addition using the substrates, i.e. peat moss , vermiculite or mixture of both on in vitro rooting characteristics and shoot length of strawberry plantlets. Data explored that cultivar Marquez with 0.5 mgl-1 IBA was the most favorable treatment for increasing rooting percentage, number of roots, and root length/ plantlet during rooting stage compared to Festival cv. As for substrates, using peat moss alone followed by the substrate consisted of peat moss+ vermiculite(1:1,v/v) were effective treatment for increasing rooting percentage, number of roots, root length and shoot length/ plantlet in in vitro rooting of the two strawberry cultivars Festival and Marquez. Fourth experiment: Was done to determine the suitable substrate for Festival and Marquez cvs growth during acclimatization stage. Data reported that culturing the transplants of the two tested cultivars in pots contained combination of peat moss+ vermiculite + sand (1:1:1, v/v/v) recorded the highest transplant survival percentage (93 to 96%), number of leaves, root number and length compared with the other used substrates.