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العنوان
Studies on biocontrol agents and genetic diversity of selected fluorescent
pseudomonads for combating pathogenic microorganisms /
المؤلف
Said, Alaa Fathalla Mohamed Farag.
هيئة الاعداد
باحث / آلاء فتح الله محمد فرج سعيد
مشرف / متولى على بركة
مناقش / راشد عبد الفتاح
مناقش / هبه محمد عبد النبي
الموضوع
Beans. Microbiology.
تاريخ النشر
2015.
عدد الصفحات
127 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم النبات
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة قناة السويس - كلية الزراعة - النبات الزراعي
الفهرس
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Abstract

The objectives of the present investigation were including five items: (a)
Isolation and screening of antagonistic fluorescent pseudomonads from root samples
of different crops. (b) Phenotypic characterization of antagonistic fluorescent
pseudomonads by biochemical traits. (c) Functional traits (extracellular fungal cell
wall degrading enzymes, secondary metabolites, plant growth promoting traits, genes
that encode specific functions of antibiotic production). (d) Genetic characterization
of antagonistic fluorescent pseudomonads by rpob. (e) Pot experiments were carried
out to evaluate the role of the efficient Pseudomonas strains in promotion of bean
plant growth and biocontrol of soil-borne pathogen. (t) Effect of different carrier
material type (rock phosphate, perlite, bentonite, vermiculite, and their combination
on populations of bacteria. A total of 200 strains of fluorescent pseudomonads were
isolated from root samples of different crops. Thirty four strains showed a broadspectrum
antifungal activity towards phytopathogenic fungi IRhizoctonia solani AG
2-2, Fusarium solani and Pythium arrhenomanes). Based on the antagonistic
potential, twenty isolates of fluorescent pseudomonads were studied In detai 1.
Antagonistic fluorescent pseudomonads were identified by molecular method to five
major groups being group ] was formed by 3 strains of Pseudomonas palleroniana
and 1 strain of Pseudomonas moraviensis, group 2 was formed by 1 strain
Pseudomonas anguilliseptica, group 3 was formed by strains, representing the major
group, 2 strains of Pseudomonas entomophila,l strain of Pseudomonas parafulva, 3
strains of Pseudomonas plecoglossicida, one strain of Pseudomonas mosselii and 5
strains of Pseudomonas putida, group 4 was formed by 1 strain of Pseudomonas
argentinensis and group 5 was formed by 2 strains of Pseudomonas corrugate.
Comparison of inoculated and non inoculated plants in infested soil showed that
antagonistic fluorescent pseudo monads gave great support to host plants. Beans
plants inoculated with antagonistic fluorescent pseudomonads were significantly
higher than all treatments. The effect of different carrier material type on populations
of bacteria appeared that vermiculite was the best carrier for Pseudomonas putida
SAW19 strain.