الفهرس 
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Abstract
Effect of Nicotinamide on Streptozotocin-Induced Diabetic Rats Treated with Bone Marrow Mesenchymal Stem Cells. Eman Gouda Mohamad, master degree, Biochemistry and Nutrition Department, Faculty of Women for Arts, Science and Education, Ain Shams University.
The main objective of this work is to assess the role of nicotinamide in protecting β-cells of ratʼs pancreas after STZ injection and to study the therapeutic potency of bone marrow mesenchymal stem cells in STZ-induced diabetic rats. Ten male albino Wister rats were sacrificed and used for isolation and differentiation of MSCs into insulin producing cells within 4 weeks. Fifty male albino rats were divided into 5 groups. group (1): normal control. The other groups were injected with STZ 50 mg/kg b.w. for induction of diabetes. group (2): diabetic control, group (3): diabetic rats received oral nicotinamide (NIC) 100 mg/kg b.w. daily (NIC group), group (4): diabetic rats injected subcutaneously with differentiated MSCs 5x106 cells per rat (MSCs group), group (5): diabetic rats injected subcutaneously with differentiated MSCs 5x106 cells per rat and received oral NIC 100 mg/kg b.w. daily (MSCs + NIC group), the treatment continued for 4 weeks.
The results indicated that injection with MSCs and administration of oral NIC ameliorate the glucose homeostasis by decreasing blood glucose and increasing insulin and C-peptide levels, improving oxidant-antioxidant status by decreasing serum MDA and TOC levels and increasing the activity of liver SOD and GPX. Serum lipid profile (TC, TAGs, LDL-C and VLDL-C) were deceased while HDL-C was increased. Using both of MSCs and NIC caused more improvement which was evidenced in the histopathological examination of pancreatic cells. ¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬¬In conclusion: treatment with nicotinamide improves the diabetic state of rats as nicotinamide is believed to have anti-hyperglycemic and anti-oxidant properties. MSCs were successfully differentiated into numerous numbers of β-cells and injection with 5×106 MSCs clearly support pancreas tissue repair.