الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
FMDV cause a serious contagious transboundary viral disease affecting
cloven hoofed animals leads to huge economic losses. It is classified into 7 immunologically distinct serotypes, 0, A, C, Asia 1, SATI, SAT2 and SAT 3.
Genetic variation in FMDVs occur due to changes in the genes encoding
capsid proteins resulted in antigenic difference and require vaccine matching
studies for antigenic characterization and proper selection of the vaccine.
Therefore, the present study was conducted for molecular
characterization and phylogenetic analysis of circulating FMDV strains
during 2014 to assure the vaccine efficacy used in Egypt.
The FMDV genomic RNA was extracted from collected samples to be
used in detection of FMDV in suspected infected cattle by rR T - PCR using
general primer targets 3D gene which used as the primary tool for the FMDV
detection directly from collected samples without need for virus isolation. The rRT- PCR was not designed to differentiate between FMDV
serotype as it was designed for highly conserved regions in the FMDV
genomic (3D gene), so the mRT-PCR was employed on positive rRT-PCR
FMDV samples to determine the circulating FMDV serotypes. The results of mRT-PCR revealed that FMDV strains of three serotypes 0, A and SAT2
were detected using specific primers targets VPl gene.
Comparative alignment of partial VPl gene sequence of identified
FMDV strains (O-EISharqyia-Egy-2014, A- EISharqyia-Egy-2014 and
SAT2-EIDaqahlia-Egy-2014) was performed with other genotype-defined
FMDV strains which is useful for the identification of the circulating virus
genotype that important for selection of the vaccine to improve FMD control.
In addition to analysis of deduced aa sequence ofVPl gene was performed on
studied FMDV strains which is of predominant importance to localize the
sites of aa substitutions, whether it have been occurred within important antigenic sites affecting the recognition of FMDV by host Mabs.