Search In this Thesis
   Search In this Thesis  
العنوان
The Role of Uroplakin IIIA (UPIIIA)
Gene and its Protein in the Diagnosis of
Bladder Cancer
المؤلف
Mohamed,Fatma Mahmoud Abdelwahed
هيئة الاعداد
باحث / Fatma Mahmoud Abdelwahed Mohamed
مشرف / Ahmed Osman Mostafa
مشرف / Nadia Iskandar Zakhary
مشرف / Samy Ramzy Shehata
مناقش / Ahmed Osman Mostafa
الموضوع
qrmqk Bladder Cancer.
تاريخ النشر
2015.
عدد الصفحات
257p;
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
الكيمياء الحيوية ، علم الوراثة والبيولوجيا الجزيئية
تاريخ الإجازة
1/1/2015
مكان الإجازة
جامعة عين شمس - كلية العلوم - الكيمياء الحيوية
الفهرس
Only 14 pages are availabe for public view

from 257

from 257

Abstract

The Role of Uroplakin IIIA (UPIIIA) Gene and its Protein in the Diagnosis of Bladder Cancer.
Background: Advanced bladder cancer is an aggressive malignancy with a poor prognosis. Despite precise pathologic staging and grading, prediction of clinical outcomes in patients is very difficult. Uroplakins are urothelial differentiation-related membrane proteins, they represent major urothelial cyto-differentiation products and are highly conserved during mammalian evolution.
Objective: To evaluate the role of uroplakin IIIA as a potential diagnostic and / or prognostic predictor marker for metastasis of bladder cancer patients.
Methods: A total of 106 subjects: 61 bladder cancer patients, 20 benign cases, 25 healthy subjects, were enrolled in the present study. Patients were observed for 3 years postoperative. UPIIIA mRNA level was detected in blood using q-PCR, and in urine by conventional PCR, while urinary UPIIIA protein was measured using ELISA.
Results: At baseline, before cystoscopy, blood UPIIIA mRNA wassignificantly higher in the metastatic patients as compared with controls, patients with benign lesions, and nonmetastatic cancer ones (p< 0.015, 0.02, and 0.03; respectively). Urinary UPIIIA protein was significantly higher in patients who developed metastasis within the 3 years observation period from the time of surgery.
Conclusion: The results suggest that UPIIIA may provide a feasible non-invasive tool for differential diagnosis and prediction of metastasis in bladder cancer.
Key words: bladder cancer, UPIIIA, metastasis.