الفهرس 
HyperlipidemiaOnly 14 pages are availabe for public view
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Abstract
Throughout this study a number of 72 healthy male albino rats weighing (140±5.6) g were subjected to experimentation. Hypercholesterolemia was induced by cholesterol at (10 g /kg diet) and solid fat (50 g/kg diet) the experiment contained six groups, each group consists of 12 rats as follow; group (1) Served as control, fed on balanced diet (without treatment);groups (2) and (3), rats were fed on balanced diet containing 10% barley and malted barley respectively, group (4), rats were fed on HFHC (50 g solid fat +10 g cholesterol/kg diet) to induce hypercholesterolemia. Groups (5) and (6), rats were fed on HFHC contains 10% barley and malted barley respectively. Balanced diet freely was offered to all groups and water ad libitum for 6 weeks. The results illustrated that:
1- It is observed that the rats fed on a diet containing high fat high cholesterol caused a highly significant increase in all studied lipids parameters except that HDL-C, The increment reached 27.22 %, 40.18%, 49.74%, 61.21%, 49.75%, 72.28% and 72.97%for TL, TC, TAG, LDL-C, VLDL-C, AI and risk factor respectively in high fat high cholesterol as compared to control group. On the other hand, HDL-C was significantly (P<0.05) decreased in high fat high cholesterol rats it recorded 36.25±2.91 as compared to control rats. High fat high cholesterol group treated with barley and malted barley reflected a significant depletion in all parameters of lipid profile and the decrement reached 22.04 % 24.87%, 25.19 %, 40.34 %, 34.11 %, 54.37 %, 54.89 % for Tl, TC, TAG, LDL-C, VLDL-C, AI and risk factor for high fat high cholesterol group co-treated with barley when compared with high fat high cholesterol group.
On the same manner the decrement reached 33.95%, 36.05%45.31%, 60, 23%, 45.33%, 73.77% and74.93% for Tl, TC, TG, LDL-C, VLDL-C, AI and Risk factor for high fat high cholesterol group co-treated with malted barley when compared with high fat high cholesterol group with an exception of good cholesterol (HDL-C) showed a significant increase in the mean value to reach (47.62±2.44), (57±5.70) for high fat high cholesterol groups co-treated with barley and malted barley respectively when compared with high fat high cholesterol group (36.25±2.91).
2- With regard of antioxidant status and lipid peroxidation the results revealed that the level of reduced glutathione in blood recorded 25.77± 3.65 this value decreased significantly to reach 16.86 ± 2.52 in high fat high cholesterol group. While, the rats co-treated with barley and malted barley showed a significant increase in the level of reduced glutathione that reach 22.87±1.51 and 24.14±4.04 respectively when compared with high fat high cholesterol group (P< 0.05) and the level of serum MDA level in control group recorded 3.89±0.334 this value increased significantly to reach 5.87±1.13 in high fat high cholesterol group while, the rats treated with barley and malted barley caused a significant decrease in the serum level of MDA which recorded 4.09±0.63 and 3.54±2.30 respectively for high fat high cholesterol co–treated with barley and malted barley respectively when compared with high fat high cholesterol group.
3- Antioxidant status and lipid peroxidation in liver tissue, the results illustrated that the results demonstrated that the rats fed on high fat high cholesterol diet showed a significant decrease in the level of reduced glutathione and superoxide dismutase activity in liver tissue which recorded 14.66±4.22 and 162.39±5.53 respectively when compared with control groups.
4- The results also revealed that high fat high cholesterol caused a significant increase in serum level of ALT and AST activities than control group and high fat high cholesterol treated with barley and malted barley. Serum level of ALT and AST increased by 221.4% and 198.4% respectively in group of rats fed high fat high cholesterol diet when compared with control group. Also, there were a significant decrease in serum activities of ALT and AST in groups treated with barley and malted barley than high fat high cholesterol group, so the percentage of change in serum activity of ALT was 32.67 % and 34.45 % in groups of rats fed on barley and malted barley respectively. Also, the percentage change in serum activity of AST was 37.23 % and 43.18% in groups of rats fed barley and malted barley respectively (P<0.05).