الفهرس 
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Abstract
This work was carried out to study the zoonotic importance of Cronobacter sakazakii as a potential foodborne emerging pathogen involved in severe illness and deaths in humans, especially neonates.
To achieve this purpose, the following points were inducted:
6.1 Occurrence of Cronobacter spp. in different samples collected from Zagazig city, Sharkia province, Egypt:
• A total of 420 samples [PIF (n=100), raw milk (n=100), milk powder (n=20), human infant stool (n=100) and animal feces (n=100)] were collected and subjected to bacteriological examination for the presence of Cronobacters.
•Sixteen samples out of 420 examined (3.8%) were found positive for Cronobacter spp.
•The isolation rates were 5% in PIF, 7% in animal feces and 4% in infant stool, 0% in each of milk powder and raw milk samples.
•The identity of the isolated organism was confirmed as Cronobacter spp. by subjecting the bacteriologically positive samples to PCR technique using 16S rRNA species specific primers.
•Cronobacter specific 16S rRNA was detected respectively in 2/5, 6/7 and 4/4 of bacteriologically positive PIF, animal feces and infant stools examined.