الفهرس 
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Abstract
Enteric bacterial infections in poultry pose a threat to intestinal
health and can contributed to poor feed efficiency and livability of a
flock.
Salmonella are among the major bacterial pathogens causing huge
economic losses to the poultry industry, as well as posing a significant
threat to public health. Avian salmonellosis is a problem of economic
concern to all phases of poultry industry from production to marketing.
Another serious poultry pathogen and zoonotic bacterial agent is P.
aeruginosa. It affects newly hatched chickens drastically causing high
mortality and mass death of embryos.
The objective of this study was todetect the microbial RNA, which
is less stable than DNA, in clinical samples of diseased chickens as an
indicator for the expression of potential virulence properties of bacterial
agents.
Herein,144 samples of each intestines, lungs and liver were
collected from diseased chickens from various Egyptian chicken farms
(68) and examined by conventional culture methods for isolation and
identification of some Gram negative bacteria.
By conventional culture method, E. coli was highly recorded
(49.3%)from all examined chicken cases, followed by Proteus spp.
(16.67%), other bacterial species were also recorded such asShigella spp.
(5.56%), Klebsiella spp. (4.86%), Salmonella spp. (2.78%) and
Pseudomonas spp. (1.39%).
Bacteriological examination of chicken farms suffered from
respiratory manifestations in various Governorates in Egypt showed that
E. coli was recorded in 23 out of 68 examined farms with a percentage of
33.82% followed by Proteus species (29.41%), Shigella spp.(7.4%),.