الفهرس 
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Abstract
This study was planned to explore the potential association among several clinical variables and some digestive troubles in buffalo including stomatitis, acute traumatic reticuloperitonitis, acute rumen acidosis and enteritis, address the hemato-biochemical alterations as well as the measurement of selected acute phase proteins including haptoglobin (HP) and C- reactive protein (CRP) in the diseased buffalo, evaluate the potential significance and the link between gene expression of selected pro inflammatory and anti-inflammatory cytokines as well as immunoglobulins in the diseased buffalo using RT-PCR. The present study included 50 native breed water buffalo, 40 of them were suspected to have digestive disorders and were allocated to four equal groups: the first group included animals with stomatitis (n = 10), the second group included buffalo with acute TRP (n = 10), the third group included buffalo with acute rumen acidosis (n = 10); while the last one included animals with enteritis (n = 10). Ten apparently healthy water buffalo were also randomly selected and considered as a control group. The investigated animals were raised at different farms and different localities in Dakahlia governorate, Egypt. The animal`s body weight ranged between 350 to 450 kgs and age ranged between four to five years. Ten mL of blood was collected from each animal via jugular vein puncture and was divided into three aliquots. The first aliquot was added to a plain tube for obtaining blood serum for estimation of the following parameters: ALP, AST, ALT, Ca, Mg, Na, K, Fe, total protein, albumin, blood urea nitrogen, glucose, CRP and HP. The second and the third aliquots were collected in tube containing EDTA for hematological examination and PCR assays. The results of real time PCR revealed a significant up regulation of IL-1β, IL-6, IL-10, and TNFα gene expression level in blood of diseased buffalo compared with those of controls; while the results of qPCR revealed a significant down-regulation of both IgG and IgA gene expression level in blood of diseased buffalo compared with controls.