الفهرس 
DIABETES M ELLITUSOnly 14 pages are availabe for public view
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Abstract
Objective: Osteopontin (OPN) is a sialoprotein implicated in different immunity and metabolic pathways. OPN plays a significant role in the development/progression of several autoimmune diseases; interestingly, it was also shown to participate in the acute pancreatic islets response to experimentally-induced diabetes in NOD mice. Furthermore, OPN promotes adipose tissue dysfunction, systemic inflammation and insulin resistance.
Aims of this study: To evaluate circulating Osteopontin (OPN) levels in patients with Type 1 DM in comparison to healthy controls and to explore clinical and biochemical correlates of OPN concentrations in those patients and its association with carotid intima media thickness (CIMT) as a marker of subclinical carotid atherosclerosis.
Design: Cross sectional study.
Methods: We enrolled 60 children and adolescents with type 1 diabetes mellitus and 30 healthy sex and age-comparable controls.
Study population: Underwent medical history taking, clinical evaluation, blood sampling for biochemistry and screening for diabetes complications. Serum OPN levels were measured by enzyme linked immunosorbent assay (ELISA).
Carotid intima media thickness (CIMT) as a marker of subclinical carotid atherosclerosis.
Results:
T1DM patients had significantly higher serum OPN levels than controls (5 (4.75 – 10) v.s 1.3 (1 – 1.5) µg/l, p=0.000). OPN levels in T1DM were positively correlated with disease duration (p<0.01), diastolic blood pressure, albumin excretion rate and Carotid intima media thickness (CIMT) (p<0.05).
OPN levels were significantly increased in patients with micro-vascular complications (p=0.000).
T1DM patients had significantly increased carotid intima media thickness (CIMT) compared to control group (p=0.000) with 21.7% had increased CIMT (p=0.006).
CIMT was significantly increased in patients with micro-vascular complications (p=0.009). 67.6% of patients with micro-vascular complications had increased CIMT (P=0.022).
Analysis of OPN levels in relation to CIMT revealed significant higher levels among patients with increased CIMT than those with normal CIMT (p=0.014).
Conclusions: This Cross sectional study demonstrated that patients with T1DM had increased serum OPN levels and CIMT than controls and higher OPN concentrations are associated with an unfavorable metabolic profile and positive micro-vascular complications in these patients.