الفهرس 
Histology of Normal Thin SkinOnly 14 pages are availabe for public view
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Abstract
Introduction: Skin aging is a complex biological process. Photoaging describes the sun damaging effects on skin mainly due to chronic ultraviolet (UV) light exposure. To date, currently available anti-aging strategies have unsatisfactory results for the patients which generate the urge for new treatments. As adipose-derived stem cells (ASCs) are available in abundant quantities, harvested by a minimally invasive procedure, could be safely transplanted and differentiated along multiple cell lineages, their use in treatment of many diseases has extensively been investigated.
Aim of The Work: To assess the potential ability of ASCs to ameliorate skin changes in adult female guinea pigs induced by chronic exposure to artificial light source similar to the sun rays in its UVA and UVB spectrum.
Materials and Methods: Adipose-derived stem cells were isolated from subcutaneous white adipose tissue of five adult human donors undergoing elective liposuction surgery. Twenty adult female guinea pigs were used and were randomly divided into two groups, each was subdivided into two subgroups five animals each. Subgroup IA served as control. Subgroup IB was intradermally injected with phosphate buffered saline solution. Subgroup IIA served as the photoaging model. Subgroup IIB served as the photoaging model intradermaly injected with ASCs. Isolated stem cells were cultured, characterized and differentiated into osteogenic lineage. Skin specimens were prepared and examined using different histological and immunohistochemical techniques. Morphometric and statistical studies were also performed.
Results: Subgroup IIA showed many UV damaging effects in the skin epidermis and dermis, while ASCs injection in subgroup IIB resulted in partial restoration of the skin epidermis and dermis structures.
Conclusion and Recommendation: Intradermal injection of ASCs partially improved the photodamaging effects. Thus, further studies are needed before ASCs injections could be clinically used to treat photoaging.