الفهرس 
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Abstract
The current study was designated for isolation and identification of Rotavirus in calves as one of the most important and main viral infection causing diarrhea in newborn calves in El-Gharbia province in Egypt by testing 50 fecal samples of diarrheic calves below three months of age from October 2015 till March 2016 by PCR and FAT, In addition to evaluation of Entero-3 vaccine in late pregnant caws by SNT. by using reverse transcription polymerase chain reaction (RT-PCR),out of the 50 fecal samples examined only 5 samples were identified as positive (10%). The samples were inoculated in MDBK cells revealed successful isolation of rotavirus in four samples with characteristic cytopathic effects showed rounding of cells, shrinkage of cell wall together with an increase of granularity and progressed to form a bunch of grapes within 72-96 hrs and show the characteristic green apple fluorescence by FAT, In addition to increase Ab titer against rotavirus at time of parturition and in the newly born calves in compare with controlled unvaccinated caws.