![]() | Only 14 pages are availabe for public view |
Abstract Newcastle disease (ND) is a highly contagious disease affects several avian species, responsible for devastating outbreaks in commercial poultry flocks. Despite application of various chickens’ vaccination programs, the disease appears in an endemic form in commercial broiler and layer poultry farms. This study was conducted to identify the prevalent NDV strain responsible for sever outbreaks in broiler poultry farms in ElGharbiya Governorate. Twenty pathological specimens were inoculated in embryonated chicken eggs 9-11 days via allantoic sac revealed hemorrhagic dead embryos 72 hrs after third passage. Successful amplification of (400bp) of the fusion (F) protein by RT-PCR in 15 out of 20 tested filed isolates (75%). In addition, phylogenetic analysis based on a partial sequence of the F protein gene clustered these isolates within class II, genotype VII velogenic Newcastle disease viruses with Identity percentage of (94.2-97.7 %) with NDV-chicken /China / SDWF07l2011. Key words: -NDV, RT-PCR, F protein, genotype VII, and phylogenetic alignment |