الفهرس 
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Abstract
Newcastle disease (ND) is a highly contagious disease affects several
avian species, responsible for devastating outbreaks in commercial poultry
flocks. Despite application of various chickens’ vaccination programs, the
disease appears in an endemic form in commercial broiler and layer poultry
farms. This study was conducted to identify the prevalent NDV strain
responsible for sever outbreaks in broiler poultry farms in ElGharbiya
Governorate. Twenty pathological specimens were inoculated in
embryonated chicken eggs 9-11 days via allantoic sac revealed hemorrhagic
dead embryos 72 hrs after third passage. Successful amplification of (400bp)
of the fusion (F) protein by RT-PCR in 15 out of 20 tested filed isolates
(75%). In addition, phylogenetic analysis based on a partial sequence of the
F protein gene clustered these isolates within class II, genotype VII
velogenic Newcastle disease viruses with Identity percentage of (94.2-97.7
%) with NDV-chicken /China / SDWF07l2011.
Key words: -NDV, RT-PCR, F protein, genotype VII, and phylogenetic alignment