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Abstract Otitis media (OM) is one of the most prevalent illnesses of infancy and early childhood. It is the most leading cause of conductive hearing loss in children, and speech/language delay that associated with adverse social and educational outcomes. Mothers play the most significant role not only in providing care for their children suffering from otitis media, but also in preventing this disease which leads to improve quality of life of their children. This study aimed to assess mothers’ practices of knowledge about care of their children suffering from otitis media, and to develop a health education module for them. A descriptive design was used to conduct this study at the outpatient clinics of Ear, Nose and Throat of Mansoura University Hospital. The data was collected from a convenient sample of 127 mothers whose children were under 5 years old and suffering from otitis media, with and without complications of otitis media. Data about mothers’ knowledge and their practices about care of OM were collected using a structured questionnaire sheet. Results of this study revealed that, 63.8% of the studied children their age were ranged from 1 year to less than 3 years, as well as, there was a positive relation between mothers’ knowledge and their practices to provide care for their children suffering from otitis media. This study concluded that, more than half of studied mothers had ”insufficient” knowledge score and ”unsatisfactory” score in practical knowledge regarding otitis media. |