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العنوان
Haematological, Biochemical and Histological characteristics for Some fish species from the Red sea /
المؤلف
Abd El-Awad, Ola Ibrahim Muhammad.
هيئة الاعداد
باحث / علا ابراهيم محمد عبد العواض
مشرف / أسامة محمد محمود
مناقش / نصر الله حسن عبد الحميد
مناقش / عبد الله بكر محمود
الموضوع
Fish - Physiology.
تاريخ النشر
2017.
عدد الصفحات
142 p. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم الحيوان والطب البيطري
الناشر
تاريخ الإجازة
24/5/2017
مكان الإجازة
جامعة أسيوط - كلية العلوم - Zoology Department
الفهرس
Only 14 pages are availabe for public view

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from 142

Abstract

In the present study specimens of the Red Sea seabream (Diplodus noct), the Red Sea goatfish (Parupeneus forsskali) and the Klunzinger’s wrasse (Thalassoma klunzingeri) were captured from the Red Sea at Hurghada, Egypt to study their Haematological, biochemical and histological characteristics. The results can be summarized in the following points: A. Haematological characteristics 1. The RBCs count is highest in Diplodus noct followed by Thalassoma klunzingeri and Parupeneus forsskali. 2. Hb and Hct levels and erythrocyte in¬dices (MCV, MCH, and MCHC) are highest in Parupeneus forsskali and lowest in Thalassoma klunzingeri. B. Biochemical characteristics 1. The levels of AST and ALT are highest in Thalassoma klunzingeri followed by Diplodus noct and Parupeneus forsskali. 2. The total protein level is highest in Thalassoma klunzingeri followed by Parupeneus forsskali and Diplodus noct. 3. Glucose level is highest in Parupeneus forsskali followed by Diplodus noct and Thalassoma klunzingeri. C. Protein analysis by polyacrylamide gel electrophoresis (SDS - PAGE) 1. The protein bands of Thalassoma klunzingeri, Diplodus noct and Parupeneus forsskali were 4, 4 and 6 respectively. 2. Based on cluster analysis Diplodus noct and Parupeneus forsskali were more similar species than Thalassoma klunzingeri. D. Blood cells characterization 1. Erythrocytes, thrombocytes, neutrophils and lymphocytes were identified in the blood smears of the three species studied. 2. Eosinophils are found only in the blood of Diplodus noct. 3. Monocytes are found only in the blood of Parupeneus forsskali and Thalassoma klunzingeri. 4. Immature erythrocytes are found only in the blood of Parupeneus forsskali and Thalassoma klunzingeri. 5. Dividing erythrocytes are rarely observed only in Thalassoma klunzingeri. E. Morphometric of the blood cells 1. Erythrocyte cell volume is largest in Thalassoma klunzingeri followed by Parupeneus forsskali and is smallest in Diplodus noct. F. Cytochemistery of blood cells 1. Only neutrophils of the three species studied and eosinophils of Diplodus noct are stained by Periodic Acid-Schiff (PAS). 2. Bromophenol blue (BB) stains only the erythrocytes of the three species, neutrophils and eosinophils of Diplodus noct. 3. Lymphocytes, monocytes and thrombocytes of the three species are not stained by PAS or by BB. G. Histology 1. The liver of the three species studied is composed of a parenchyma covered by a delicate and thin capsule; which composed of a layer of simple squamous epithelial cells and a thin layer of loose connective tissue. 2. In Diplodus noct the parenchymal hepatocytes lie in anastomosing laminae or cords around the central vein. 3. In Parupeneus forsskali and Thalassoma klunzingeri, the parenchymal hepatocytes are radially arranged around the central vein in interconnecting laminae or cords of two cells thickness. 4. The pancreatic exocrine tissue is distributed in the liver of Diplodus noct and Thalassoma klunzingeri as hepatopancreas but not detected in the liver of Parupeneus forsskali. 5. The kidneys of the studied species are covered by a connective tissue capsule and are composed exclusively of hematopoietic tissue which fills among the nephrons. Each nephron consists of glomerulus that is enclosed by Bowman’s capsule, the proximal, distal and collecting tubules.