الفهرس 
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Abstract
The present study was conducted in the department of Animal Physiology, Faculty of Veterinary Medicine Zagazig University, and Animal Reproduction & Artificial Insemination Department, Veterinary Division, National Research Center (NRC), Dokki. Giza, Egypt. The samples was collected from Sharkawy Abattoir, Bahteem, Cairo during the period from March 2014 until July 2015.
A total number of 360 ovarian pairs from non-pregnant cattle with an average live body weight of 400-500 kg were used in the present study. Healthy buffalo-cows ovaries were collected, separated shortly after slaughtering of animal, dissecting away from the surrounding tissues and maintained in a thermos flask containing the transport medium (sterile normal saline solution added with 100,000 U/ml penicillin and 100 mg/ml streptomycine at 30-35 C for 1-2 hours.
In the laboratory, the ovaries were washed three times with warm normal saline to remove adhering blood. Fat and other non-ovarian tissues were dissected away from the ovaries .
The follicles were classified according to its size into small (5-8 mm), medium (9-11 mm) and large follicles (12-14 mm).The corpus luteum was classified into mature, recent, corpus hemorrhagium and corpus albicans. Gene expression of Leptin hormone, gonadotropin-releasing hormone receptor (GnRHR) and Inhibin hormones with different sizes of follicles and stages of corpus luteum was recorded by using reverse transcription-PCR technique. Leptin, oestradial-17 and progesterone hormone concentrations in the follicular fluid and.