الفهرس 
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Abstract
The aim of this study was to estimate the levels of Leptin and TNF-α in serum and gingival crevicular fluid, in patients with chronic periodontitis, before and after phase I therapy. Patients and Methods : A total of thirty subjects were included in this study; 20 systemically healthy chronic periodontitis patients (group I) and 10 systemically healthy subjects with clinically healthy gingiva as a control group (group II). The nonsurgical periodontal therapy was done in group I. Periodontal parameters, including plaque index, gingival index, bleeding on probing, probing depth and the clinical attachment level, were recorded. GCF and serum were collected from all individuals included in the study ; the first sample was collected from all groups (study and control) before phase I therapy. The second sample was collected 4 weeks after phase I therapy from group I. Levels of Leptin & TNF-α in GCF and serum were quantified using ELISA.Results were analyzed statistically using computer program SPSS. The results: - showed a statistically significant reduction in total levels of leptin & TNF-α in serum and TNF-α in GCF in study group after phase I therapy. In contrast, leptin levels in GCF of study group showed a significant increase after phase I therapy. Whatever the levels of leptin and TNF-α in serum or GCF were increased or decreased after non-surgical treatment these levels were directed toward normal levels of the control group. Results also showed all clinical parameters were significantly improved after the phase I periodontal therapy in groups I (p<0.001). Conclusions:1- Scaling and root planing (SRP) is the mainstay of treatment of periodontal diseases as SRP was effective in improving clinical parameters in patients with chronic periodontitis. 2- Leptin & TNF-α could be used as a potential diagnostic marker for periodontal disease activity in both serum and gingival crevicular fluid.