الفهرس 
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Abstract
Infectious bursal disease virus (IBDV) is a cause of immunosuppressive disease of young chickens causing economic losses to the poultry industry worldwide. It continues to be a serious problem in Egypt. In this study, recent outbreaks of IBDV in broiler and layer chicken flocks in Sharkia Province, Egypt during 2016 were investigated. The main symptoms were depression, closed eyes, trembling, shivering, anorexia, prostration, vent picking, whitish watery diarrhea. Grossly, bursa was hyperemic and enlarged covered with cream colored transudate. Hepatic infarction and swollen kidneys with accumulation of urates with severe dehydration were seen.
Bursal samples were collected and subjected to total RNA extraction and real-time RT-PCR with primers specific to the VP2 gene to detect viral RNA in this tissue. The IBDV was detected in 10 out of 16 bursa tissues tested with a percentage of 62.5%. It was detected in 7 (77.8%) and 3 (42.9%) samples collected from broiler and layer chickens, respectively. Regarding to vaccination, the IBDV was detected with a percentage of 64.3% and 50% in vaccinated and non-vaccinated flocks, respectively. Among the examined chicken breeds, the Baladi (native breed) was less affected, with a percentage of 50%, followed by Sasso and Cobb breeds (66.7%). The most affected breeds were Bovins and Rush with a percentage of 100%.