الفهرس | Only 14 pages are availabe for public view |
Abstract Isolation and identification of some amylolytic fungi isolated from soil of Dakahlia governorate. Using some agriculture wastes i.e. wheat bran, rice bran, corn bran and potato waste for amylase production by the most active fungal isolates via solid state fermentation technique Optimization of amylase production by the most active four fungal isolates grown on wheat bran medium. Studying factors affecting amylase activity of the fungal amylases. Methods & Results : Eight amyolytic fungal isolates were isolated from different soil in Dakahlia governorate and identified. The isolates were identified and belonged to Penicillium sp.(1), Penicillium sp.(2), Penicillium sp.(3), Penicillium chrysogenum, Penicillium georgiense, Verticillium sp., Alternaria sp. and Aspergillus westerdijkiae. After 7 days of incubation the maximum production of amylase by Penicillium sp .(1) was 267.92 Au/g waste with WB, the maximum yield of amylase by Penicillium sp.(2). 268.96 Au/g waste was obtained with WB after 5 days, the maximum yield of amylase by Penicillium chrysogenum (398.47 Au/g waste) was obtained with wheat bran after 7 days, the highest values of amylase (407.65 Au/g waste ) after 7 days by Penicillium georgiense and Penicillium sp.(3). at the optimum time of incubation was 320.49 Au/g waste, by Verticillium sp. the maximum production of amylase was 258.6 Au/g waste, by Aspergillus westerdijkiae (387.45 Au/g waste ) was obtained at the 7th day of incubation and the highest value of amylase (194.57 Au/g waste) was obtained from Alternaria sp . at the 7th day of incubation when WB was used. Conclusion: Amylase is one of the most widely used enzymes for the production of fermented foods and starch, and the demand for amylase is increasing with a widening spectrum of applications. |