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Abstract 200 fish (100 Mugil cephalus, 100 Nile tilapia) collected randomly from Port-Said markets for Pseudomonas species isolation. (66%)Mugil and (80%)Tilapia were positive for Pseudomonas presence. Bacteriological examination of different organs revealed the presence of 436 Pseudomonas isolates; Pseudomonas fluorescens (29.13%), Pseudomonas aeruginosa (27.06%), Pseudomonas putida (11.01%), Pseudomonas cepacia (10.55%), Pseudomonas stutzeri (8.49%), Pseudomonas anguilliseptica (5.73%), Pseudomonas alcaligenes (4.13%) and Pseudomonas acidovorans (3.90%). PCR revealed that 14 tested isolates were Pseudomonas, 7 of them were Pseudomonas fluorescens and 4 were Pseudomonas aeruginosa.All isolates were sensitive to Ciprofloxacin, Gentamycin and Chloramphenicol and resistant to Penicillin, Amoxicillin and Ampicillin/sulbactam. |