الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Atotal of 314 samples (82 of each of liver and intestine) collected from
diseased broiler in 2013 and(75 of each of liver and intestine) collected
from diseased broiler in 2014 were examined bacteriology for the
prevelance of C. perfringes . The recorded isolates were subjected to
biochemical tests( catalase, sugar fermentation, gelatin liquefaction,
litmus milk and indoletests), Nagler`s test, dermonecrotic reactions in
albino guinea pigs which proved that all the recorded isolates were
C.perfringens types A, D and non toxigenic, toxin antitoxin
neutraliation test.Clostridium perfringens was isolated from 132
samples , 91 (66.93%) of them were toxinogenic that subtyped into
type A 75 (57.57%) and typeD 15 (11.36%) by intradermal inoculation
in G.pig.The higher incidence of C.perfringens was recorded in winter
(57.5% in 2013 and 53.12% in 2014) autumn (50% in 2013 and 50% in
2014) then summer (37.93%) in 2013 and 32.60% in 2014) and the
lower incidence was recorded in spring (31.25% in 20013 and 30% in
2014). Isolation rate of Clostridium perfringens from intestine and liver
samples of diseased chicken in 2013 and 2014 was 65.85% and 58.66%
,respectively from intestine and 21.95 % and 21.33% ,respectively from
liver.PCR protocol used for amplification and detection of alpha and
epsilon toxin gene of 6 isolates of Clostridium perfringens .profiling of
the alpha and epsilon toxin proteins by sodium dodecyl sulphate
polyacrylamide gel electrophoresis (SDS-PAGE). The most effective
antibiotic was Recommended for treatment of NE was applied by
sensitivity test to 91 isolates of toxinogenic Clostridium perfringens
The histopathological changes of intestine and liver samples of the
diseased birds with N.E was disscused in details.