الفهرس 
Introduction and aim of the workOnly 14 pages are availabe for public view
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Abstract
Introduction: Bone defects occur after tumor resection, post trauma, or in developmental deformities. Diseased bones do not heal properly and this leads to reduced quality of life with social and psychological problems. Bone-tissue engineering had emerged as an alternative method to autologous or allogeneic bone grafts.
Aim: to evaluate the efficacy of ADM versus propylene mesh both loaded with BM-MSCs in healing of skull bone defect in adult albino rats.
Materials and methods: the study included 40 male albino rats. Five weaned male albino rats were used for preparation of BM-MSCs. Five adult rats were used to obtain the dermal graft. Thirty adult rats weighing 150- 200 gm were divided into three groups according to the way of filling skull bone defect. group I: Ia (-ve control), Ib (spontaneous healing). group II: IIa (unseeded propylene), IIb (seeded propylene). group III: IIIa (unseeded ADM), IIIb (seeded ADM).. The trephine operation was done on the left parietal bone. Specimens were collected four weeks postoperative and processed for H&E, osteopontin immunohistochemistry and scanning electron microscope. Morphometric and statistical analysis were also performed.
Results: bone defects filled with seeded scaffolds showed significant progression in new bone formation than unseeded scaffolds. Seeded grafts showed a significant increase in the mean percentage osteoblastic surface, mean thickness of osteogenic layer at the edge of the defect and area percentage of osteopontin expression. Seeded ADM showed significant increase in histomorphometric parameters compared to seeded propylene mesh.
Conclusions: It was concluded that seeded ADM provides strong support for new bone formation in clavarial bone defect.
Key words: bone defect, propylene mesh, acellular dermal graft, histology, osteopontin, rat