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Abstract SUMMARY AND CONCLUSION Two storage experiments were carried out during the two successive summer plantation seasons of 2013/2014 and 2014/2015 at the Department of Natural Products, National Research Center for Radiation Research and Technology, Nasr City, Cairo, Egypt. The purpose of this experiment was minimizing post-harvest losses in potato tubers (Salanum tuberosum L.) using gamma radiation, mint oil, paclobutrozole and their relates under unrefrigerated storage condition. Two cultivars of potato were used, Diamont (Semi-late maturing tolerates loss) and Sponta (early maturing and sensitive to loss). All suitable agriculture practices were taken into consideration, i.e. cultivation, chemicals treatments of the propagation material, irrigation, maturing and fertilization, harvesting, and handling. Irradiation process was performed using Indian unit of Cobalt-60, the radiation dose was 100 Gy at the dose rate og 28.5 Gy/h and 27.9 Gy/h in the two successive seasons. Tubers were soaked in 5% mint oil, 100 ppm of paclobutrozole and combination of the different treatments among radiation, mint oil and paclobutrozole were performed. All treatments were performed under the temperature ranged at 27°C ±2 and the relative humidity was 70 - 80%. Data of storage on the physical and chemical traits of the tubers were recorded every two weeks. Treatments were: Control, 100 Gy dose of gamma rays, 5% mint oil, 100 ppm Paclobutrazole, 5% mint oil and 100 Gy of gamma radiation, 100 ppm Paclobutrazole and 100 Gy of gamma radiation, 5% mint oil and 100 ppm Paclobutrazole and 5% mint oil, 100 ppm Paclobutrazole and 100 Gy of gamma. 78 SUMMARY AND CONCLUSION Soha M. El-Sherbiny, (2017), Ph.D., Fac. Agri., Ain Shams Univ. Results on cv. Diamont could be summarized as follows: 1.Physical characters: 1.1. Weight loss: The highest weight loss % was recorded in cose of treatment with mint oil. All treatments: radiation, PP333, radiation + mint oil, radiation + PP333, mint oil + PP333 and radiation + mint oil + PP333 showed less weight loss % of tubers as control during both seasons. The best result in weight loss depression was obtained from radiation + PP333 combined treatment after 10 weeks of storage. 1.2. Sprouting: The highest sprouting % was shown by control, mint oil and mint oil + PP333. The lowest sprouting % value was observed with PP333 treatment in the two seasons. 1.3. Shrinkage: For both two seasons, the highest tubers shrinkage % was taken place by mint oil, control and followed by mint oil + PP333. 1.4. Decay: Decay starts to appear in the 8th week in control, mint oil, PP333, radiation + mint oil, mint oil + PP333 and mint oil + PP333 + radiation treatment but radiation and radiation + PP333 didn’t show any decay. 1.5. Total Loss: Total loss started at the 4th week in all treatments under study. The highest significant loss was observed in the control, mint oil and mint oil + PP333 treatments but less effect was recorded with radiation, PP333 and radiation + PP333 during both seasons. 79 SUMMARY AND CONCLUSION Soha M. El-Sherbiny, (2017), Ph.D., Fac. Agri., Ain Shams Univ. 1.6. Specific Gravity: All treatments gave high specific gravity for tubers specially those of mint oil + PP333 treatment in all periods of storage than control treatment. 2. Chemical characters of tubers: 2.1. Dry matter: All treatments had high content of dry matter over 20% with significant differences between 20% and 25% in all intervals of storage and in both seasons. 2.2. Total protein: All treatments had higher protein content than control. It was also observed that with increasing the periods of storage the protein content was decreased up to the end storage periods, in both seasons. 2.3. Total sugars: The highest content of sugars was shown for radiation + mint oil treatment compared with the other tested treatments. Sugar contents decreased gradually by increasing periods of storage up to the end of the experiments in both seasons. 2.4. Carbohydrates content: The highest significant carbohydrates % was shown by radiation + mint oil, followed by the rest of treatments. 2.5. Starch content: Significant differences in starch content of the different periods of storage in their different treatments were in a very narrow range in both seasons. 80 SUMMARY AND CONCLUSION Soha M. El-Sherbiny, (2017), Ph.D., Fac. Agri., Ain Shams Univ. 2.6. Endogenous Hormones Levels: As for GA3 levels, high significant levels were shown by control, mint oil and radiation + mint oil than other treatment, after storage. As for IAA levels, a high significant activity of IAA was shown by control, followed by mint oil, and radiation+ mint oil, after storage. As for ABA levels the highest significant level was shown by radiation, PP333 and radiation + mint oil, before storage but low levels of ABA was shown by most treatments after storage. Results on cv. Sponta Could be summarized as follows: 1. Physical characters: 1.1. Weight loss: The lowest weight loss was shown by radiation and radiation + PP333 treatments than the other used treatments. 1.2. Sprouting: Sprouting started to appear in some treatments at the 8th week and at 10th week in other treatments. In radiation and radiation + PP333 sprouting appears at 12 weeks. in both seasons. 1.3. Shrinkage: Shrinkage started at the 10th week storage in all treatments except radiation, PP333 and PP333 + radiation treatments which showed no shrinkage. 1.4. Decay: Decay started at the 6th week of storage in mint oil + PP333 treatment but at the 12th week in control and mint oil treatments. 81 SUMMARY AND CONCLUSION Soha M. El-Sherbiny, (2017), Ph.D., Fac. Agri., Ain Shams Univ. 1.5. Total loss: Total loss started at the 4th week of storage. Radiation, PP333 and radiation + PP333 showed the lowest total loss but without significant differences in the second season. 1.6. Specific Gravity g/cm3: A gradual increase in specific gravity was shown during of storage up to the end of the period in all treatments, in both seasons. It was also observed that the untreated tubers, PP333 and radiation +PP333 have the highest specific gravity in both seasons. 2.Chemical characters of the tubers: 2.1. Dry matter: All treatments showed a higher content of dry matter ranged between 19- 28% in all intervals of storage and in both seasons with significant differences among them 2.2. Protein: A gradual decrease in total protein content was observed in periods of storage in all treatments in both seasons. A significant decrease in protein content was showed by mint oil + PP333 than other used treatments in both seasons. 2.3. Total sugars: Radiation, mint oil, PP333 and radiation + mint oil + PP333 treatments recorded the highest significant total sugars in both seasons. 2.4 Carbohydrates: Radiation + PP333 and radiation +mint oil + PP333 followed by radiation treatments had higher contents of carbohydrates than other tested treatments in both seasons. 82 SUMMARY AND CONCLUSION Soha M. El-Sherbiny, (2017), Ph.D., Fac. Agri., Ain Shams Univ. 2.5. Starch %: Radiation + PP333, radiation + mint oil treatments recorded the highest starch content in comparison with other used treatments. This was true n both seasons. 2.6. Endogenous hormones levels: High levels of GA3were observed after storage than before storage. Control, mint oil, and radiation + mint oil + PP333 showed the highest levels after storage than the other treatments in the first season. Control and mint oil showed the highest IAA content than the other treatments in the first season. Radiation, PP333 and radiation + PP333 showed the highestlevels of ABA than other used treatments. Generally, low levels of ABA were shown after storage than before storage of the tubers. |