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العنوان
Studies on Gamma Rays Induced Mutations in Some Cultivated Egyptian Soybean Varieties /
المؤلف
Diab, Rana Hosny Mohamed.
هيئة الاعداد
باحث / رنا حسني محمد دياب
مشرف / عادل رمضان الشنشوري
مشرف / رضا محمد جعفر
مشرف / مروة محمود حمودة
الموضوع
Botany.
تاريخ النشر
2017.
عدد الصفحات
p 244. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علوم النبات
تاريخ الإجازة
15/10/2017
مكان الإجازة
جامعة طنطا - كلية العلوم * - Botany
الفهرس
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Abstract

The objectives of this investigation were to study the effects of moderate(150 and 200Gy) and high doses (250Gy) of Gamma radiation on some Egyptian varieties of soybean at the morphological and molecular levels and to study how much these effects are transmitted from the M1 to the M2 generation. This study also aimed at the determination of the doses of - radiation that may induce mutations, which help in developing new varieties of soybean with useful characters. Finally, this study aimed at the determination of the doses of -radiation that may enhance the salinity tolerance of two studied soybean varieties. Seeds of seven Egyptian soybean varieties (Giza21, Giza22, Giza35, Giza82, Giza83, Giza111, and Crawford) were studied at the molecular level using ISSR and SDS-PAGE techniques to evaluate their genetic variability. Then, two varieties (Giza 111 and Crawford), which are commonly cultivated in Egypt were selected and used for further mutation studies using various-radiation doses(150, 200 and 250 Gy) and M1 and M2 generations were evaluated at morphological and molecular levelst the morphological level, the measured criteria were seedling height, percentage of germination, shoot height, branching of stem, days to maturity, days to flowering, percentage of pod shattering, pod length, number of seeds per pod, number of pods per plant, crop yield and weight of 100 seeds. In addition, presence of stem appendages, leaf abnormalities, flower color, pod appendages, pod color, coat color, seed hilum color were also documented. At the molecular level, effects of exposure to different -radiation doses have been documented using SDS-PAGE and by ISSR analyses in M0, M1 and M2 generations.