الفهرس 
INTRODUCTION
Cryptococcus. sppOnly 14 pages are availabe for public view
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Abstract
Milk under the influence of its high nutritive value it is considered an excellent medium for pathogenic as well as non- pathogenic fungi such as a family of Cryptococcusand Candidaspp.
A total of two hundred raw milk samples were randomly collected including buffalo and cow (70 samples each) and sheep and goat ( 30 samples each ) from different localities in Sohag city. These samples were examined for the enumeration of total fungi,total count of yeast andtotal mold in comparison to total fungi count /ml.In addition to the occurrence of Cryptococcus and Candidaspp.the obtained results indicated that average total fungi counts were 1.05Χ105 for buffalo milk,1.21 Χ105 for cow milk,1.27 Χ105 for sheep milk and 6.40Χ104 / ml .for goat milk . The percentof total yeast count /mlwas 97.14% of total fungi , for buffalo milk ,91.73% for cows milk ,95.27% for sheep milk while ,in goat milk it was 50% of total fungi .
Using the API method revealed, the incidence of Cryptococcus and Candida spp. were 6 (8.57%) 2(2.8%) ,3(4.28%) for Cr. neoformans, Cr. laurentii and Cr. terreus in buffalo milk, however , in cow milk 5 (7.14%) for Cr.neoformans .In caseof Multiplex PCR , it was performed on 10 isolates from buffalo milk and 10 isolates from cow’s milk , and the results revealed that buffalo’s and cow’s milk were contaminated with Cr. neoformans with a percent of 30 and 20% , respectively. Concerning C. albicans which was previously identified by API, 10 samples of them gave positive results using PCR.
from the current study it was approved that there is a relation between mastitis and incidence of Cr. neoformans and C. albicans. In buffalo’s milk revealed that one sample of positive C. M. T. recorded 100% for both Cr. neoformans and C. albicans. While, in cow milk 2 samples of C. M. T. recorded 50% C. albicans. In sheep and goat there is no relation between mastitis and presence of Cyptococcus or Candida spp.
The public health hazard of the isolated organisms and the recommended hygienic measures were discussed.