الفهرس | Only 14 pages are availabe for public view |
Abstract The genera Escherichia, Klebsiella, Enterobacter, Serratia, and Citrobacter (collectively called the coliform bacilli) and Proteus include overt and opportunistic pathogens responsible for a wide range of infections. Escherichia coli is the most commonly isolated organism in the clinical laboratory. Many of these microorganisms used to be dismissed as harmless commensals. Today, they are known to be responsible for major health problems worldwide. The widespread and often indiscriminate use of antibiotics has created drug-resistant Gram-negative bacilli that readily acquires multiple resistance which has been increasingly reported worldwide and it is also one of the major causes of failure in the treatment of infectious diseases. So, scientists directed to herbal products, which may give a satisfied solution for this problem. Therefore, the main objective of this study was to determine the antibacterial effects and the action of thymol and euganol purified extracts on the virulence factors of MDR, motile and haemolytic E. coli isolates phenotypically via TEM and SDS-PAGE and genotypically on the expressions of some virulence genes of E. coli isolates recovered from equine sources using qRT-PCR. Out of 215 different types of samples collected from Sharkia and Cairo Governorates, 182 Enterobacteriaceae species were recovered with a high prevalence rate (84.7%). Seven different bacterial species were identified with E. coli and Klebsiella spp. being the predominant members among the total collected samples (33 and 28.8%, respectively). Notably, E. coli isolates represented the highest recovery rate (39%) among all Enterobacteriaceae members, followed by Klebsiella species. |