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العنوان
Phenotypic and Genotypic characterization of Salmonella spp. Isolated from Camel in North Western Coastal Area of Egypt /
المؤلف
El HELW, Marwa Fawzy ALI ALI.
هيئة الاعداد
باحث / مروه فوزي علي علي
مشرف / نشوى عبدالسلام عزالدين
مشرف / محمود درديري محمدالحريري
مشرف / محمد احمد السيد بلاطه
مشرف / اماني عبد النبي حافظ
الموضوع
Camels Egypt.
تاريخ النشر
2018.
عدد الصفحات
156 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2018
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Microbiology
الفهرس
Only 14 pages are availabe for public view

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from 192

Abstract

This study was applied on 284 samples of swabs, fecal samples and organs which were collected from living & slaughtered dromederies camels in north western coastal area of Egypt. Bacteriological and biochemical tests were applied on them, 26 samples were positive to Salmonella . The results of VITEK classified them into 22 field isolates of salmonella spp. and 4 of them were Salmonella enteric ssp diarizonae. The 26 isolates showed positive band of invA gene.12 of them were positive to spvA gene of S. Entritidis and 10 of them positive to fliC gene of S.Typhimurium. All 26 isolates were resistant to spiramycin (100%) & sensitive to ciprofloxacin, gentamicin, and amoxicillin/clavulinic. It is obvious not all resistant isolates carry the resistant gene of tetracycline tetA and β-lactam blaTEM & blaSHV & vice versa.SDS explained that the mol.weight of bands were closely related with variable heterogenous banding profile. The analysis of 16s rRNA gene of 11 isolates showed 100% similarity between 3 isolates of the same origin ,S. Entritidis and with one samples of S.Typhimurium while the percentage of divergence was 0.2- 0.4%.