الفهرس | Only 14 pages are availabe for public view |
Abstract Cucumber production exposed to huge losses in Egypt and worldwide due to infection with Cucumber mosaic Cucumovirus (CMV). The current study focus on biological and molecular identification of two Egyptian CMV isolate and the impact of one Egyptian CMV isolate (Gharbia –CMV) on metabolic and oxidative activities in cucumber plants. CMV isolates was obtained from cucarbitaceous crops and confirmed by DAS-ELISA. Inoculated cucumber plants by infectious crude sap with two CMV isolates showed difference in external symptoms. The metabolic and biochemical components in cucumber plants were affected with (Gharbia –CMV) isolate. The present study provided that, CMV infection caused a significant reduction in both photopigments and biochemical components while bioactive components (proline and phenol) and oxidative enzymes (POX, SOD, PPO and CAT) were increased significantly compared healthy ones. It was found that (Gharbia –CMV) isolate was belonging to subgroup1A and similar to other Egypt isolate while (Sharqia-CMV) isolate was belonging to subgroup 1B and similar to Iran isolat. |