الفهرس 
Cytocomptability of chitosanOnly 14 pages are availabe for public view
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Abstract
Many researchers have demonstrated that chitosan
is a natural biocompatible, non-toxic, and biodegradable
polymers which have been used broad range of
applications, in the biomedical, agricultural, technological
fields and various industries.
Chitin, was usually extracted from crustaceous
exoskeleton, recently the alternative sources take great
attention due to their enormous biodiversity and lower
inorganic materials. Through deacetylation process by acidbase
chemical reaction chitin can be converted to its
soluble derivative chitosan.
Aim of the work:
The present study is planned to extract chitin from
natural sources such as exoskeleton of different common
insect species and shell fish of Nile tilapia then their
derivatives, chitosan are prepared. The characterization
analysis of these extracted and commercial chitosan are
compared by using Fourier transform infrared (FT-IR)
spectra, X-ray diffraction (XRD), UV-Visible
spectrophotometer and calculating their degree of
deacetylation then their effects on hematological
component are evaluated.Design and methods:
Seven common species related to different categories
are selected for chitin extraction. Six widely distributed
insect species are chosen for their exoskeleton and the
seventh is the shellfish of Nile tilapia. The extracted chitin
through deproteinization by KOH and mineralization by
acetic acid become the soluble chitosan.
The physic-chemical characterization of these
extracted chitosan are studied and then compared with the
commercial crustaceous one by using Fourier transform infra
spectra (FT-IR), FT-IR, X-ray diffraction, UV-visible
spectrophotometer and calculating their degree of
deacetylation for each.
Results and conclusion:
The extracted beetle chitosan shows the highest
degree of deacetylation between the other examined insect
species but the shellfish extracted chitosan becomes the
highest (70%) when compared with the other extracted and
commercial crustaceous one. X-ray diffraction analysis to
beetle, shell fish and commercial chitosan ensured the
reverse relation between the Crystallinity and the degree of
deacetylation as lower Crystallinity related to higher degree
of deacetylation. UV-Visible spectrophotometer is done to confirm the absorbance of chitosan acetic acid solution in
the region 260-290 nanometer, increases at higher degree
of deacetylation.
The effects of beetle, shellfish and commercial
chitosan solutions on hematological components (Red
blood cells, White blood cells, Platelets, Fibrinogen plasma
protein, Prothrombin time and partial Thromboplastin time)
on venous blood samples of ten healthy persons at different
pH are studied. The results indicate that in acidic pH only
(4 and 5) there are marked decrease in the numbers of
human blood cells. These are related to the effect of the
concentration of the cationic charges at acetic media. Also
the effect of the examined chitosan on the coagulation
assay shows increasing in the value of PT and PTT. The
statistical analysis ensured the significant effects of these
results.