الفهرس 
Classification of cancerOnly 14 pages are availabe for public view
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Abstract
Cancer continues to represent the largest cause of mortality in the world, the second leading cause of death worldwide next to cardiovascular disease. The current searching for effective non-toxic,inexpensive, and suitable neoadjuvant therapy with methotrexate(MTX) to decrease MTX dosage without lowering its chemotherapeutic efficacy. In this study we investigated the antitumor effect of trehalose(TRE) on mice bearing Ehrlich ascites carcinoma(EAC)and checke whether TRE can enhance the anticancer potential of MTX. In addition, an effective specific medications in the management and treatment of cancer. As, TRE, was previously found to have(antitumor)activities,the present study was conducted to evaluate the antitumor activity of TRE with and without MTX against EAC in mice. In this experiment,
Mice were assigned into 8 groups of twenty mice were used for assessment of antitumor activity of TRE and five mice for evaluation of mean survival tim (MST)and increase of life span percentage (%ILS).The groups are group I: (Negative control group) .group II:(EAC- bearing positive control group).group III: (Trehalose control
group).group IV: (Trehalose treated group) .group V: (Methotrexate control group) group VI: (Methotrexate treated group) .group VII:(Trehalose and methotrexate control group) .group VIII: (Trehalose and methotrexate treated group) for six times day after day. Antitumor activity of TRE was monitored by measuring the survival time, counting total number of tumor cells, monitoring autophagic activity at the cellular level by flowcytometery , monitoring autophagic and apoptotic regulated genes (Caspase 3, Bec1 and Bcl2 genes ) by real time PCR , as well as the biochemical parameters as hepatic enzymes activities in serum , oxidative stress markers in liver homogenate, in addition to complete blood picture (CBC) and histological studies of all groups