الفهرس 
Cover Page Title in EnglishOnly 14 pages are availabe for public view
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Abstract
Mastitis is the most prevalent and expensive disease in dairy farms. Administering of antibiotics is a must be step in treatment of mastitis. Beta lactam antibiotics group is considered as one of the most effective antibiotics groups used in treatment of mastitis. On the other hand, uncontrolled use of these antibiotics leads to emergence of antibiotic resistance by mastitis causing bacteria especially coliform bacteria. Bacterial resistance to antibiotics of Beta lactam group is increasing worldwide threatening the public healthcare due to the limited treatment substitutions. Beta lactamase enzymes especially extended spectrum beta lactamase is responsible for the most important resistance mechanisms of the Enterobacteriacae to antibiotics of Beta lactam group. To detect these enzymes must perform: - Phenotypic identification. Genotypic identification. This study aims to investigate the prevalence of extended-spectrum β-lactamase among Enterobacteriacae isolated from mastitis cases in cattle. This study was carried out at faculty of Veterinary Medicine Alexandria University. Total of (102) Gram negative Enterobacteriacae isolated from mastitis cases in cattle. Methods of investigation included: 1- Identification of the bacterial isolates by VITEK 2 compact system 2- Phenotypic identification of Extended Spectrum Beta Lactamase Enzymes and estimation of sensitivity of these isolates against variety of different antibiotics through Disk Diffusion Sensitivity Test. 3- Identification of Extended Spectrum Beta Lactamase Enzymes through Real Time PCR. The study of the result showed that: Number of 17 (16.6%) isolates from number of Enterobacteriacae isolates from mastitis cases in cattle that recorded positive for phenotypic inspection of Extended Spectrum Beta Lactamase and number of 14 was positive for genotypic inspection of Extended Spectrum Beta Lactamase Enzymes Gene CTX-M is the most prevalent gene in the isolated Enterobacteriacae.