الفهرس 
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Abstract
This study was carried out in order to investigate the occurrence of some virulence genes of S. aureus isolated from cattle with mastitis. A total number of 133 milk samples (45 from clinical mastitis and 88 from subclinical mastitis) were collected from dairy cattle in Kafer Elsheikh and EL Gharbia Governorates, Egypt. The samples were screened for presence of S. arueus by classical bacteriological methods. A total of 41 Staphylococcus isolates were recovered from cattle with mastitis with an incidence of 30.8%. Among the isolates, 21(15.7%) of S. aureus and 20 isolates of CNS were identified phenotypically and further characterized genotypically by polymerase chain reaction PCR. All isolates were screened for detection of haemolysine type A (hla), Haemolysine type B (hlb), binding protein A (spa) and toxic shock syndrome (tsst-1) by PCR. The obtained results revealed that spa gene was detected in all Staphylococcus isolates recovered from subclinical mastitis while in clinical mastitis was detected in S. aureus and CNS isolates with an incidence of 33.3 and 50% respectively. The data in the study provided an overview on the distribution of some virulence genes related to S. aureus isolated from mastitic cattle in Egypt.