الفهرس 
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Abstract
The present study was conducted at the Genetics and Genetic
Engineering Lab, Faculty of Veterinary Medicine, Zagazig University.
The main objective of this study was to detect polymorphisms in GDF9
and BMP15 genes in Barki and Rahmani sheep.
In the present study blood samples from 100 ewes (50 for Barki
and 50 for Rhmani) were collected from a private farm at Giza
governorate. This study was aimed to determination of polymorphisms
of GDF9 gene and BMP15 gene in Barki and Rahmani sheep by
Polymerase Chain Reaction – Restriction Fragment Length
Polymorphisms (PCR-RFLP), Single strand confirmation polymorphism
(SSCP) and DNA Sequencing for detection of SNPs in the four exons of
both breeds.
Molecular genetic studies
Polymorphism of GDF9 gene
The PCR product of exon I of GDF9 gene was 712 bp, these
amplicons had been treated with HhaI enzyme which cut the 712 bp into
(120, 254 and 338) in all animals with no differences between all the
tested animal of Barki and Rahmani sheep with absence of G1 mutation.
Also SSCP result showed the same pattern revealing no differences
in the sequence of this amplicon in both breeds.
DNA sequencing had confirmed these results. Amplicon of exon II
of GDF9 gene with 713 bp had been digested by HinfI cutting it into 4.