الفهرس 
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Abstract
Background and Aim: Polymorphisms in some genes may influence the persistence of hepatitis C virus (HCV) infection, clinical outcome, HCV replication, and liver damage. This study was conducted to investigate the role of the interferon gamma (IFN-γ) gene at (+874 T/A, −764 G/C, −179 C/A) single-nucleotide polymorphisms (SNPs), its receptor (IFN-γR2) at (hCV2786067 C/T) SNP and (rs273259, A/G) of interferon induced protein 44 Like (IFI44L) in the susceptibility of Egyptian families to HCV infection with high-resolution techniques. Methods: In total, 517 Egyptian families, with 2246 subjects, were recruited to this study from the Upper and Lower Egypt governorates and were classified into three groups: 1034 patients with chronic hepatitis C virus, 108 subjects with spontaneous virus clearance (SVC), and 1104 subjects as a healthy control group. All subjects were genotyped for {(−764 G/C, rs2069707), (−179 C/A, rs2069709), (hCV27860067, C/T) and (rs273259, A/G)} SNPs of the IFN-γ gene using the allelic discrimination real-time polymerase chain reaction (AD-RT PCR) technique, but (+874 T/A, rs2430561) SNP genotyped using amplification refractory mutational system - polymerase chain reaction (ARMS-PCR) technique and was confirmed using sequence-based typing (SBT) technique. Results: The carriage of T allele of (+874) IFN-γ is a risky allele and was significantly higher in chronic hepatitis C more than other two groups (odds ratio [OR]: 2.6646, P < 0.0002). On the other hand, the C allele of (−764, rs2069707) of IFN-γ gene and G allele of (rs273259) of IFI44L gene are a protective alleles and were higher in SVC than the other two groups (OR: 0.2709, P < 0.0001). However, both (−179 C/A, rs2069709) and (hCV27860067, C/T) SNPs are not polymorphic enough to be studied in the Egyptian population. Conclusions: HCV infection is associated with the T allele of (+874 rs2430561), while SVC of HCV is associated with the C allele of (−764, rs2069707) of the IFN-γ gene and G allele of (rs273259) of IFI44L gene.