الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 116 |  |  |
| | | from | 116 | | |
Abstract
This study aimed to detect biochemical parameter LC3 to monitor autophagy in Vitiligo skin of diseased patients as compared to normal control persons to investigate the possible role of autophagy in the pathogenesis of this disease.
This case control study was conducted in dermatology outpatient clinic at Beni-Suef University hospital in the period from (1st November 2017 to 31 of May 2018). The study included sixty individuals divided into two matched groups of 30 Vitiligo cases and 30 healthy controls that were chosen randomly according to inclusion and exclusion criteria.
All the sixty individuals included in the study was subjected to a written informed consent for participation in the study, detailed history taking, clinical assessment to determine type, extent and sites of Vitiligo. Then all participants underwent four mm punch skin biopsy will be taken from patients (Vitiligo lesion) & control and it was kept in lysis solution for the stability of the studied parameters and was kept frozen at -80 Celsius till analysis of autophagy protine LC3 by real time -PCR.
The collected data were coded then entered and analyzed taking three months using the SPSS version 22.
The study revealed that the average patient age was 33.73 ±8.6 (SD) years and gender distribution as 76.7% of them were females and 23.3% were males with no statistically significant difference between cases and control groups regarding age and sex. The analysis of autophagy protine LC3 in the punch skin biopsy from our studied population (patients with vitiligo lesion & control skin) reveled that; level of LC3 in lesional skin of vitiligo patients was significantly lower as compared to normal control persons (p-value < 0.001); where the mean scores were (0.37 ±0.3 vs. 1.03 ±0.1) in cases and controls respectively.