الفهرس 
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Abstract
Jaundice occurs in most newborn infants. Most jaundice is benign, but because of the potential toxicity of bilirubin, newborn infants must be monitored to identify those who might develop severe hyperbilirubinemia and, in rare cases, acute bilirubin encephalopathy or kernicterus.(AAP,1994)
Antioxidants levels remain low at birth; however, this induces a series of RBC breakdown and heme metabolic processes. There is an association between bilirubin concentrations and reductive and oxidative reactions (Chou.H,et al,2014).
Aim of the work :
The aim of the present study was to predict severity of Neonatal Hyperbilirubinemia by measuring cord blood Hydrogen peroxide level.
Subject of the method :
This was a Case –control study conducted at the fifty term neonates, who were delivered at EL-Minia maternity &children’s university hospital during the period from June 2016 to October 2016 they were followed up for development of neonatal Jaundice.
Forty-one neonates developed neonatal jaundice, fourteen of them didn’t follow up while 27 continued follow up and were assigned as group 1.
Nine neonates didn’t develop neonatal jaundice and were considered as control group 2.
Patients consisted of 27full term neonates ,13(48%) male and 14(51.9%) female whose mean gestational weight was 2.6± 0.4kg
All individuals in this study were subjected to full clinical assessment and complete physical examination. Complete blood count, Reticulocyte count, Maternal and neonatal blood group &RH serum bilirubin (total and direct) of cord blood for both groups and serum bilirubin follow up at 5th,7th day of life for group 1 &2, and Measurement of serum hydrogen peroxide level from cord blood sample for both groups and from venous blood sample postnatal at the 5th day and 7th day for group 1 only by using Human hydrogen peroxide (H2O2) ELISA Kit
Results:
we found that significantly higher levels of bilirubin in cord blood at 5th ,7th day more than in control with peak level at 5th day and mean SD 13.8 md/dl± 2.9.
Level of hydrogen peroxide have significant higher levels at 5th ,7th day more than in control with peak level at 5th day (P value<0.001) and mean SD 128 pg/ml± 37.7.
Also we found that a significant positive strong correlation between cord H2O2, 5th ,7th and cord,5th ,7th respectively bilirubin (r=0.817& p<0.001), and there was a highly significant correlation between cord H2O2 and bilirubin at 5th day (r=0.466& p<0.014)
We found that Sensitivity of hydrogen peroxide = 92.6.%, Specificity= 100%, Cut off point >35 as predictor of neonatal hyperbilirubinemia
Conclusion:
We found Reach to conclusion that Hydrogen peroxide levels and bilirubin concentrations in cord and neonatal blood are closely related and Cord blood level of Hydrogen peroxide can predict neonatal hyperbilirubinemia.
Lastly we recommended that Further large studies are required to confirm the diagnostic and prognostic and possible role of Hydrogen Peroxide in predication of neonatal hyperbilirubinemia.