الفهرس | Only 14 pages are availabe for public view |
Abstract Red sea was recognized as a rich source of microbial diversity with unique metabolites. Marine fungi are known to produce a great variety of secondary metabolites with unique chemical structures that have appealing bioactivities. The present study was aimed to screen for different biological activities in Sponge and Soft corals associated fungi of the Red Sea coast at Hurghada and evaluate the effect of media composition on the production of active secondary metabolite. In this context, four marine sponge and coral associated fungi [Aspergillus sydowii SP52-EGY, Aspergillus tamarii SP73-EGY, Aspergillus sp. 2C1-EGY and Aspergillus ungusSPMD-EGY] were isolated, identified and registered in Gene Bank. Applying the OSMAC (One Strain Many Compounds) technique in this study, the four isolated fungi were grown on four different media (A,B,C &D) under shake and static conditions and the different biological activities (antimicrobial, antioxidant, enzyme inhibitors and anticancer activity) of the crude ethyl acetate extracts of secondary metabolites were evaluated. The extracts of the four fungi demonstrated variable biological activity in-vitro assays. The secondary metabolite extracts of the four fungi were tested against a panel of three cancer cell lines, HCT-116, HepG2 and MCF-7 and the results revealed significant cytotoxic activity of the extracts. Extracts of the four sponge and coral associated fungi; which showed high cytotoxic activity in the monolayer cell culture model (2D),were selected for further investigation in the three-D multicellular spheroid (MCS) model (3D).Two active extracts in the 2D model (A St F and D St F) showed significant cytotoxic activity on the (3D MCS) for the fungus A. SPMD. The chemical composition of both extracts were analyzed by GC/MS spectroscopy and showed totally different chemical composition. It was concluded that secondary metabolites of sponge and soft corals associated fungi of the Red Sea coast considered as potential producers of bioactive compounds that have antimicrobial, antioxidant and anticancer activities. |