الفهرس 
Common enteropathogensOnly 14 pages are availabe for public view
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Abstract
Diarrheal disorders are a major health problem in pediatrics worldwide. Accounting for more than 750,000 deaths in children under the age of 5 per year, they are the second leading cause of death in this population according to the World Health Organization. In Egypt dairrhea is the first cause of infant mortality. Definitions of diarrheal episodes are usually based on the duration of symptoms rather than etiology. The most commonly recognized definition of diarrhea is based on World Health Organization parameters and define diarrhea by increase in frequency and /or fluidity of stool relative to the previous habit of the individual. Diarrhea may be either due to infectious or non infectious causes, so the aims of this study were: to find out various enteropathogens causing acute diarrhea in children and determine the antibiotic sensitivity and resistance among various bacterial isolates.
During this study 120 stool samples isolated from children with acute diarrhea (<14 days) underwent the following:
1. Macroscopic and microscopic examination.
2. Preparation of wet smear.
3. Detection of fecal leucocytes using methylene blue stain.
4. Carbol-fuchsin stain for the rapid detection of Campylobacter.
5. Detection of Rota virus using rapid detection immune-chromatography.
6. Culture of samples on mackongey agar, XLD and incubation at 37 for 24 hours.
7. Identification of isolates was done by;
Colony morphology.
Gram stained film Biochemical reactions as (citrate – urease – indole – oxidase -production of acid and gas on triple sugar iron agar) and motility testing.
Verification of results using Api 20E for enterobactericae for lactose, xylose non fermenting colonies.
8-Isolation and Identification of E.coli (EPEC) by serotyping using polyvalent anti sera (O26,55,111,119,126) .
9-Detection of O157:H7 E.coli using sorbitol mackongey media.
10-The isolates obtained were tested for antimicrobial sensitivity over mueller hinton agar by Kirby Bauer-disk diffusion method.
10-ESBL detection by modified double disk synergy test (MDDST).
The Results:
A higher incidence of cases was detected in rural areas (64.2%) than urban areas (35.8%).
Bacterial enteropathogens were more common (70.8%) with predominant EPEC (46.7%) followed by Salmonella (14.2 %) then viral diarrhea by Rota virus represented (20.8%).
Viral diarrhea was mainly at age of 1.5 years (61.3%). Bacterial causes, E.coli was the predominant particularly at 2 years old )82.1%) followed by Salmonella, Shigella at age of 4 years old (85%) & (10%) respectively. Parasites were mainly at age of 2.5 years (24.4%).
Bottle feeding was significantly associated with all cases of viral diarrhea (n=25) while junk food was significantly associated with bacterial diarrhea caused by EPEC, Salmonella and Shigella (n=63).