الفهرس 
AcknowledgementOnly 14 pages are availabe for public view
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Abstract
Dental pulp stem cells (DPSCs) are considered an easily accessible source of mesenchymal stem cells holding great promise for use in tissue repair and regenerative medicine. In order for DPSCs to be used in therapeutic clinical applications, issues like safely enhancing culture expansion need to be addressed. Objective: In this research, we aimed to assess the safety of platelet rich plasma (PRP) as a promoter of proliferation in comparison to routinely used animal derived supplements. Methods: The effect of PRP on the proliferation of DPSCs was assessed by MTT assay. Expression of stemness- related genes OCT4 & INTGRIN1 was analyzed by real- time quantitative PCR. DNA sequencing was performed for OCT4 & INTGRIN1 genes to ensure that the isolated DPSCs stem cell properties were not altered by the PRP supplemented media. Results: At a concentration of 1% with platelet count of 1.5 x 106/cm3, PRP was able to significantly increase the proliferation rate while maintaining the viability of DPSCs in comparison to routinely used 15% FBS. Mesenchymal stem cell surface markers expression (CD29, CD105) were not altered by PRP supplementation. Moreover, PRP in cultures significantly promoted expression of stemness markers OCT4 & INTGRIN1 compared with 10% FBS. The results were confirmed by DNA sequencing for OCT4 & INTGRIN1 genes where DPSCs cultured in PRP demonstrated 100% matching with the control group indicating that PRP supplementation didn’t alter the nucleotide sequence of stem cell genes. Conclusions: Taken together, our results indicate that PRP is a safe and efficient promoter of proliferation and can readily substitute traditional animal derived supplements like FBS.