الفهرس 
PsoriasisOnly 14 pages are availabe for public view
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Abstract
Background and Aim: Psoriasis is a chronic debilitating disease affecting1-2% of the white population. It is characterized by recurrent episodes of red, scaly, raised skin plaques that develop within seemingly normal skin and is triggered by a large number of factors, such as drugs, stress, physical injury to the skin and infection. Our aim was to detect the expression of serum and tissue (lesion and non-lesion) of LncRNA MALAT-1 and MiRNNA-9 used as biomarkers for psoriasis.
Subject and Methods: Blood samples were taken from 20 psoraiatic patients as well as 20 controls. 4 mm punch biopsy was taken from each lesional and non lesional skin of psoriasis patient and controls. The following were done: An informed consent, history taking, and clinical assessment of the disease: extent of disease (%), disease severity: using
PASI score and assessment of the severity of the biopsied plaque (BPSS).
Serum was separated for detection of: LncRNA MALAT-1 and
miRNNA-9 expressions by real time RT-PCR.
Results: The results revealed significant increase in the expression of MALAT-1 in lesional and non-lesional skin and serum of psoriatic patients than controls. Also there was statistical significant increase in serum MiRNA-9 in patients than controls. Meanwhile, its tissue level was significantly decreased in patients than controls.
Conclusion: This study highlights the contribution of MALAT-1and miRNA-9 in the pathogenesis of psoriasis. Elevated expression of MALAT-1 in lesional psoriatic skin in comparison to non-lesional skin is probably an important factor in the development of psoriatic plaques.
Key words: Psoriasis, MALAT-1, MiRNA-9 and PCR