الفهرس 
INTRODUCTION & AIM OF THE WORKOnly 14 pages are availabe for public view
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Abstract
In the present study, the physicochemical properties and the oxidative stability of Egyptian corn germ, soybean and sunflower oils produced by aqueous enzymatic extraction using a fungal cellulase from Trichoderma reesei, a bacterial protease from Bacillus licheniformis and an animal protease from bovine pancreas (Type I), either individually or in combination were compared to that extracted by hexane. An optimization of oil extraction using the different enzymes was carried out to maximize the oil yield. The optimized conditions for producing maximum oil yield from corn germ and soybean seeds using the newly used bovine protease, were 0.1 and 1% enzyme concentration, respectively, 15 and 20% seed concentration, respectively, pH 7.5 and incubation time of 60 and 240 min, respectively versus 0.63 and 0.8%, 15 and 20%, pH 8 and 120 and 240 min, respectively for bacterial protease, while those of sunflower oil extracted by bacterial protease were 0.79% enzyme concentration, 20% sunflower seed concentration, pH 8 and incubation time of 120 min. The maximum corn germ oil yield (62.38%) was obtained by combining cellulase with bacterial protease relative to hexane extraction (100%). Also, the highest yield of soybean and sunflower oils (73.04 and 66.47 %, respectively) was obtained by bacterial protease relative to hexane extraction (100%).The refractive index, iodine, saponification and peroxide values, DPPH• scavenging activity, as well as fatty acids composition of enzymes and solvent extracted-oils were comparable. The color index of the enzyme extracted- corn germ and soybean oils was lower than that of hexane extracted- oils. Also, the acid value of enzyme extracted- corn germ and sunflower oils was lower than that of the hexane extracted- oils indicating that the enzymes could be used in the production of eco-friendly vegetable oils.