الفهرس 
Introduction & Aim of the workOnly 14 pages are availabe for public view
 |  | from | 105 |  |  |
| | | from | 105 | | |
Abstract
Acne vulgaris is a chronic inflammatory disease of the pilosebaceous unit and is among the most common dermatological conditions worldwide. Acne vulgaris is a multifactorial disease. Resistin is an adipokine which is believed to play a role in inflammatory diseases. We aimed to investigate Resistin gene polymorphisms in acne patients and the relation between the gene polymorphisms and serum lipid levels.
This case-control study was included 100 subjects; who were recruited from the Outpatient Clinic of the Dermatology, Andrology & STDs Department, Minia University Hospital , and selected every other one over a period of 6 months (from the beginning of January 2017 to the end of June 2017). Subjects were divided into 2 equal groups: group I: Fifty patients diagnosed as acne vulgaris & group II: Fifty healthy, age and sex matched volunteers with no apparent systemic or dermatological diseases served as control subjects. Global Acne Grading system was used to assess the severity of acne. Total cholesterol, triglycerides, Low density lipoprotein-cholesterol and HDL-cholesterol; fasting lipid levels were determined. PCR–RFLP technique was used to detect polymorphisms of RETN +299G>A and RETN –420 C>G.
The patients group included 19 males and 31 females, their age ranged from 16 and 27 years and mean age of 19.7 ± 2.6. The prevalence of mild and moderate degrees of acne vulgaris among patient group was 38%, 48 % respectively, while 14 % suffered from severe acne. Twenty patients had a negative family history, while 30 had a positive family history. Mean of BMI for patients was 24.7±2.6. The control group included 19 males and 31 females, their age ranged between 15 and 27 years and mean age of 19.7 ± 2.6. No statistically significant difference was found between patients and control as regard age and sex. Thirty five of control had a negative family history, while 15 had a positive family history. Family history was significantly more frequent among patients than controls. Mean of BMI for controls was 24.6±3.5. No statistically significant difference was found between groups as regard BMI.
RETN gene polymorphisms at -420C > G genotype distribution showed a significant difference between patients and control groups in CG & GG genotypes. Subjects carrying G allele were significantly more likely to develop acne vulgaris. There was a significant difference between patients and control groups in frequency of distribution of GA & AA genotypes in RETN gene polymorphisms at +299G > A. There was a significant difference between female patients and female control in frequency of distribution of CG & GG genotypes RETN -420C > G polymorphism whereas; in male there was no significant difference. for RETN +299G > A polymorphism in the female There was a significant difference between patients and control groups in frequency of distribution of GA & AA genotypes, There was no significant difference between male study groups.
There was a significant difference in lipid profile between two groups. There were significant positive correlations between both RETN gene polymorphisms and GAG, severity, TC & LDL. There was a significant negative correlation between RETN polymorphism and HDL. There was no significant correlation between it and duration of disease & TG.
Study found that the RETN gene polymorphisms at -420C > G and +299G > A are significantly associated with acne vulgaris. These results additionally boost that RETN may play a focal role in acne pathogenesis due to its effects on both lipid serum levels and severity of disease.