الفهرس 
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Abstract
The current work was planned to evaluate un traditional treatments for controlling Clostridial infection in broiler chickens and comparing their efficacy with the traditional treatments as ampicillin with special references to the growth performance and the associated biochemical and hematological changes and studying the effects of such treatments on cellular and humeral immune response as well as detecting the histopathological changes.
Growth performance evaluation: Then weekly weighed till the end of experiment and the amount of feed consumed was calculated all over the experimental period. Blood samples collection and preservation: Blood samples were collected on 1st, 7th and 14th days post treatment. On 23th, 30th and 37th days of age, 5 chicks from each group were used in each collection. Two blood samples were collected from the wing vein:
1) The first blood sample was collected with anticoagulant in 2 test tubes. a) First one was collected in test tubes containing heparin 50 I.U. /ml blood as anticoagulant to determine phagocytic activity, phagocytic index. b) Second one was collected in test tubes containing EDTA 50 I.U. /ml blood as anticoagulant to measure hematological parameters.
2) The second blood sample was collected in a centrifuge tube and left to clot then centrifuged at 3000 rpm for 10 minutes to allow serum separation which then collected in Eppendorf tubes and stored at -20˚C to be used in the evaluation of different biochemical parameters, cellular and humeral immunity. The results revealed that: Clinical symptoms, Mortality rate and Lesion Scores. on 14th day of age and received Sodium butyrate and Sanguranine Phytobiotic for 5 successive days showed milder signs and mortality rate. Effect on growth performance. Effect on blood picture: A significant decrease in leukocytic count, heterophil, esinophiles basophils and monocyte allover the experimental period post treatment. Effect on some serum biochemical parameters (liver enzymes and kidney function tests): A significant increase in liver and kidney function comparing with control group. But broiler chickens infected with C.perfringens showed a significant increase in AST, ALT, ALP, uric acid and creatinine allover the experimental period. Effect of on phagocytosis %, phagocytic index, nitric oxide and Heamagglutination inhibition titers against Newcastle disease vaccine (H.I. test): A significant decrease in phagocytic activity %, phagocytic index and nitric oxide throughout experimental period post treatment when compared with infected non-treated broiler chickens. Effect on Protein picture: Ampicillin treatment showed non-significant increase in total protein albumin, A/G ratio, total globulins, alpha, beta and gamma globulins compared with non-infected non-treated group. Histopathological changes: Experimentally infected chickens with C.perfringens demonstrated liver, kidney and intestinal lesions.