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العنوان
Advanced studies on Enterococcus species recovered from poultry /
المؤلف
Saad, Aalaa Samir Ahmed.
هيئة الاعداد
باحث / الاء سمير احمد سعد
مشرف / كاميليا محمود عثمان احمد
مشرف / جيهان مصطفى بدر
الموضوع
Enterococcus. Virulence (Microbiology). Poultry.
تاريخ النشر
2019.
عدد الصفحات
299 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
البيطري
تاريخ الإجازة
1/1/2019
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Microbiology
الفهرس
Only 14 pages are availabe for public view

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Abstract

Out of 150 samples 38 chicken samples (7 brains, 23 livers and 8 hearts), 41 pigeon samples (6 livers and 35 swabs), 41 duck samples (15 brains, 20 livers and 6 hearts) and 30 human samples (30 Urine samples). The prevalence of Enterococcus isolates were 79 %, 100%, 85.4% and 66.7% in the chickens, pigeons, ducks and human samples respectively. Enterococcus spp. showed gelatin hydrolysis activity positive in 50% of Enterococcus spp. recovered from chicken, While was positive in a percentage of 34.1% , 25% and 11.4% for Enterococcus spp. recovered from pigeons, human and ducks respectively. Enterococcus spp. isolated from pigeon had the highest casein hydrolysis activity 61% followed by Enterococcus spp. isolated from chicken 53.3% then Enterococcus spp. isolated from ducks 42.9% and the lowest casein hydrolysis activity was Enterococcus spp. isolated from human source 10%. The Enterococcus spp. results for affinity percentage from Chicken were 70% and 30%, Pigeon 61% and 39%, Ducks 68.6% and 31.4% and Human 95% and 5% as moderate and strong biofilm forming capability respectively. All Enterococcus isolates supernatant fluid showed cytopathic effect on the Vero cell line (100%).The prevalence of the total γ-hemolysis activity of Enterococcus spp. reached 66.7% as well as, the β-hemolysis activity and α– hemolysis reached 29.4% and 4% respectively. The percentage of 63.5% for Enterococcus isolates were positive for Haemagglutination (HA) of sheep blood while the percentage of 28.6 % was positive for HA of chicken blood. The results showed that Enterococcus spp. of chickens origin high resistant to Clindamycin, Oxytetracycline, Doxycycline, Gentamycin(LLA), Ciprofloxacin and Vancomycin. Enterococcus spp. of pigeons origin high resistant to Clindamycin, Oxytetracycline, Doxycycline, Gentamycin(LLA) and Vancomycin . Enterococcus spp. of ducks origin high resistant to Clindamycin, Erythromycin, Gentamycin(LLA) and Vancomycin. While Enterococcus spp. of human origin high resistant to Ampicillin, Clindamycin, Oxytetracycline, Gentamycin(LLA) and Vancomycin. The results revealed that a positive amplification of the vanA, vanB and vanC genes in the 126 isolates of Enterococcus spp. were detected in a percentage of 19.84%, 16.67 and 8.73% respectively. While the a positive amplification of the catA, catB, fexA, fexB and cfr genes in the 126 isolates of Enterococcus spp. were 27%, 18.25 %, 11.90% and zero% respectively.